RNA-Seq of single prostate CTCs implicates noncanonical Wnt signaling in antiandrogen resistance-Reference-Cited by-同舟云学术

RNA-Seq of single prostate CTCs implicates noncanonical Wnt signaling in antiandrogen resistance

Published:2015-09-18 Issue:6254 Volume:349 Page:1351-1356
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Miyamoto David T.¹²,Zheng Yu¹³,Wittner Ben S.¹⁴,Lee Richard J.¹⁴,Zhu Huili¹,Broderick Katherine T.¹,Desai Rushil¹,Fox Douglas B.¹,Brannigan Brian W.¹,Trautwein Julie¹,Arora Kshitij S.¹⁵,Desai Niyati¹⁵,Dahl Douglas M.¹⁶,Sequist Lecia V.¹⁴,Smith Matthew R.¹⁴,Kapur Ravi⁷,Wu Chin-Lee¹⁵,Shioda Toshi¹,Ramaswamy Sridhar¹⁴,Ting David T.¹⁴,Toner Mehmet⁷,Maheswaran Shyamala¹⁸,Haber Daniel A.¹³⁴

Affiliation:

1. Massachusetts General Cancer Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

2. Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

3. Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.

4. Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

5. Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

6. Department of Urology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

7. Center for Bioengineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

8. Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

Abstract

Circulating signals of drug resistance Cancer drugs often lose their effectiveness because tumors acquire genetic changes that confer drug resistance. Ideally, patients would be switched to a different drug before tumor growth resumes, but this requires early knowledge of how resistance arose. Miyamoto et al. have developed a non-invasive method to spot resistance by sequencing RNA transcripts in single circulating tumor cells (CTCs) (see the Perspective by Nanus and Giannakakou). For example, in prostate cancer patients, drug resistance was triggered by activation of the Wnt signaling pathway. But CTCs are rare and fragile, and the technology needs further development before it is used in clinical practice. Science , this issue p. 1351 ; see also p. 1283

Funder

NIH

Department of Defense

Howard Hughes Medical Institute

National Cancer Institute

NCI

National Institute of Biomedical Imaging and Bioengineering (NIBIB)

Burroughs Wellcome Fund

Prostate Cancer Foundation

Johnson & Johnson

Massachusetts General Hospital

Stand Up to Cancer