Transcription polymerase–catalyzed emergence of novel RNA replicons-Reference-Cited by-同舟云学术

Transcription polymerase–catalyzed emergence of novel RNA replicons

Published:2020-04-10 Issue:6487 Volume:368 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Jain Nimit¹²³^ORCID,Blauch Lucas R.⁴^ORCID,Szymanski Michal R.⁵⁶⁷^ORCID,Das Rhiju⁸^ORCID,Tang Sindy K. Y.⁴^ORCID,Yin Y. Whitney⁵⁶^ORCID,Fire Andrew Z.¹²^ORCID

Affiliation:

1. Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.

2. Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.

3. Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.

4. Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, USA.

5. Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 77555, USA.

6. Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.

7. Intercollegiate Faculty of Biotechnology of the University of Gdansk and Medical University of Gdansk, 80-307 Gdansk, Poland.

8. Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.

Abstract

Revisiting replicating RNAs DNA-dependent RNA polymerases are well known for their ability to produce RNA from DNA templates. Much less is known about their noncanonical activity: the generation and replication of RNA from RNA templates. Deeper insights into this process could shed light on questions relating to the origin of life, molecular evolution, and the replication of certain RNA pathogens such as hepatitis delta virus and plant viroids. Jain et al. explore in detail how the bacteriophage T7 RNA polymerase generates and amplifies diverse RNA sequences in vitro. Using sequencing, microfluidics, and bioinformatics, they chart the emergence and evolution of replicating RNA motifs and identify mechanisms that explain their selection and structures. Science , this issue p. eaay0688

Funder

National Science Foundation

National Institutes of Health

Stanford University

National Science Centre, Poland

Jane B. Kempner Postdoctoral Fellowship

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference66 articles.

1. An RNA that Multiplies Indefinitely with DNA-Dependent RNA Polymerase: Selection from a Random Copolymer