Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade-Reference-Cited by-同舟云学术

Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade

Published:2021-06-11 Issue:6547 Volume:372 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Berry Sneha¹²³^ORCID,Giraldo Nicolas A.⁴^ORCID,Green Benjamin F.¹²⁵,Cottrell Tricia R.⁴^ORCID,Stein Julie E.⁴^ORCID,Engle Elizabeth L.¹²⁵,Xu Haiying¹²⁵,Ogurtsova Aleksandra¹²⁵,Roberts Charles²⁵,Wang Daphne⁵^ORCID,Nguyen Peter⁵,Zhu Qingfeng⁴^ORCID,Soto-Diaz Sigfredo²⁵,Loyola Jose²⁵^ORCID,Sander Inbal B.⁵,Wong Pok Fai⁶,Jessel Shlomit⁶^ORCID,Doyle Joshua⁷⁸,Signer Danielle⁵^ORCID,Wilton Richard⁷⁸^ORCID,Roskes Jeffrey S.⁷⁸^ORCID,Eminizer Margaret⁷⁸^ORCID,Park Seyoun¹⁹^ORCID,Sunshine Joel C.⁵^ORCID,Jaffee Elizabeth M.¹²³^ORCID,Baras Alexander⁴^ORCID,De Marzo Angelo M.³⁴^ORCID,Topalian Suzanne L.²¹⁰^ORCID,Kluger Harriet¹¹,Cope Leslie¹²¹²^ORCID,Lipson Evan J.¹²³^ORCID,Danilova Ludmila¹²¹²^ORCID,Anders Robert A.¹²⁴,Rimm David L.⁶^ORCID,Pardoll Drew M.¹²³^ORCID,Szalay Alexander S.¹⁷⁸^ORCID,Taube Janis M.¹²⁴⁵^ORCID

Affiliation:

1. The Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD 21287, USA.

2. Bloomberg~Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21287, USA.

3. Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

4. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

5. Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

6. Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA.

7. Department of Astronomy and Physics, Johns Hopkins University, Baltimore, MD 21218, USA.

8. Institute for Data Intensive Engineering and Science, Johns Hopkins University, Baltimore, MD 21218, USA.

9. Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

10. Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

11. Division of Medical Oncology, Department of Medicine, Yale University School of Medicine, New Haven, CT 06510, USA.

12. Division of Biostatistics and Bioinformatics, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Abstract

Astronomy accelerates tumor imaging Immunohistochemical stains for individual markers revolutionized diagnostic pathology decades ago but cannot capture enough information to accurately predict response to immunotherapy. Newer multiplex immunofluorescent technologies provide the potential to visualize the expression patterns of many functionally relevant molecules but present numerous challenges in accurate image analysis and data handling, particularly over large tumor areas. Drawing from the field of astronomy, in which petabytes of imaging data are routinely analyzed across a wide spectral range, Berry et al. developed a platform for multispectral imaging of whole-tumor sections with high-fidelity single-cell resolution. The resultant AstroPath platform was used to develop a multiplex immunofluorescent assay highly predictive of responses and outcomes for melanoma patients receiving immunotherapy. Science , aba2609, this issue p. eaba2609

Funder

National Institutes of Health

National Cancer Institute

Bristol-Myers Squibb

Melanoma Research Alliance

Harry J. Lloyd Charitable Trust

Sidney Kimmel Comprehensive Cancer Center

Bloomberg~Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University

the Emerson Collective

Moving for Melanoma of Delaware

the Barney Family Foundation

The Laverna Hahn Charitable Trust