The Composite Genome of the Legume Symbiont Sinorhizobium meliloti-Reference-Cited by-同舟云学术

The Composite Genome of the Legume Symbiont Sinorhizobium meliloti

Published:2001-07-27 Issue:5530 Volume:293 Page:668-672
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Galibert Francis¹,Finan Turlough M.²,Long Sharon R.³⁴,Pühler Alfred⁵,Abola Pia⁶,Ampe Frédéric⁷,Barloy-Hubler Frédérique¹,Barnett Melanie J.³,Becker Anke⁵,Boistard Pierre⁷,Bothe Gordana⁸,Boutry Marc⁹,Bowser Leah⁶,Buhrmester Jens⁵,Cadieu Edouard¹,Capela Delphine¹⁷,Chain Patrick²,Cowie Alison²,Davis Ronald W.⁶,Dréano Stéphane¹,Federspiel Nancy A.⁶,Fisher Robert F.³,Gloux Stéphanie¹,Godrie Thérèse¹⁰,Goffeau André⁹,Golding Brian²,Gouzy Jérôme⁷,Gurjal Mani⁶,Hernandez-Lucas Ismael²,Hong Andrea³,Huizar Lucas⁶,Hyman Richard W.⁶,Jones Ted⁶,Kahn Daniel⁷,Kahn Michael L.¹¹,Kalman Sue⁶,Keating David H.³⁴,Kiss Ernö⁷,Komp Caridad⁶,Lelaure Valérie¹,Masuy David⁹,Palm Curtis⁶,Peck Melicent C.³,Pohl Thomas M⁸,Portetelle Daniel¹⁰,Purnelle Bénédicte⁹,Ramsperger Uwe⁸,Surzycki Raymond⁶,Thébault Patricia⁷,Vandenbol Micheline¹⁰,Vorhölter Frank-J.⁵,Weidner Stefan⁵,Wells Derek H.³,Wong Kim²,Yeh Kuo-Chen³⁴,Batut Jacques⁷

Affiliation:

1. UMR6061-CNRS, Laboratoire de Génétique et Développement, Faculté de Médecine, 2 avenue du Pr. Léon Bernard, F-35043 Rennes cedex, France.

2. Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4K1.

3. Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.

4. Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

5. Universität Bielefeld, Biologie VI (Genetik), Universitätsstrasse 25, D-33615 Bielefeld, Germany.

6. Stanford Center for DNA Sequencing and Technology, Stanford, CA 94305, USA.

7. Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, UMR215-CNRS-Institut National de la Recherche Agronomique (INRA), Chemin de Borde Rouge, BP 27, F-31326 Castanet Tolosan Cedex, France.

8. GATC Biotech AG, Jakob-Stadler-Platz GmbH 7, D-78467 Konstanz, Germany.

9. Unité de Biochimie physiologique, Université Catholique de Louvain, Place Croix du Sud 2, Bte 20, B-1348 Louvain-la-Neuve, Belgium.

10. Unité de Biologie Animale et Microbienne, Faculté des Sciences Agronomiques de Gembloux, Avenue Maréchal Juin 6, B-5030 Gembloux, Belgium.

11. Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.

Abstract

The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain nitrogen compounds by bacterial reduction of dinitrogen (N 2 ) to ammonium (NH 4 + ). We present here the annotated DNA sequence of the α-proteobacterium Sinorhizobium meliloti , the symbiont of alfalfa. The tripartite 6.7-megabase (Mb) genome comprises a 3.65-Mb chromosome, and 1.35-Mb pSymA and 1.68-Mb pSymB megaplasmids. Genome sequence analysis indicates that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution. The genome sequence will be useful in understanding the dynamics of interkingdom associations and of life in soil environments.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference22 articles.

1. Sequence accession numbers: chromosome (EMBL CON entry); pSymA accession: (GenBank); pSymB accession number (EMBL).

2. Annotation tools and supplementary data are available at

3. Kaneko T., et al., DNA Res. 7, 331 (2000).

4. Freiberg C., et al., Nature 387, 394 (1997).

5. Gottfert M., et al., J. Bacteriol. 183, 1405 (2001).

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