Reconstitution of DNA Segregation Driven by Assembly of a Prokaryotic Actin Homolog-Reference-Cited by-同舟云学术

Reconstitution of DNA Segregation Driven by Assembly of a Prokaryotic Actin Homolog

Published:2007-03-02 Issue:5816 Volume:315 Page:1270-1274
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Garner Ethan C.¹²³⁴,Campbell Christopher S.¹²³⁴,Weibel Douglas B.¹²³⁴,Mullins R. Dyche¹²³⁴

Affiliation:

1. Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.

2. UCSF/UCB Nanomedicine Development Center, San Francisco, CA 94158, USA.

3. Physiology Course, Marine Biological Laboratory, Woods Hole, MA02543, USA.

4. Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.

Abstract

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/ parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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