Affiliation:
1. Laboratory of Molecular Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892–4255, USA.
Abstract
Eukaryotes use adenosine triphosphate (ATP)–dependent chromatin-remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP
6
) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakisphosphate (IP
4
) and inositol pentakisphosphate (IP
5
). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP
4
, IP
5
, and IP
6
impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
307 articles.
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