Generation of ovarian follicles from mouse pluripotent stem cells-Reference-Cited by-同舟云学术

Generation of ovarian follicles from mouse pluripotent stem cells

Published:2021-07-16 Issue:6552 Volume:373 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Yoshino Takashi¹^ORCID,Suzuki Takahiro²³^ORCID,Nagamatsu Go¹^ORCID,Yabukami Haruka²,Ikegaya Mika²,Kishima Mami²,Kita Haruka¹,Imamura Takuya¹⁴^ORCID,Nakashima Kinichi¹^ORCID,Nishinakamura Ryuichi⁵,Tachibana Makoto⁶^ORCID,Inoue Miki⁷,Shima Yuichi⁷⁸⁹^ORCID,Morohashi Ken-ichirou⁷⁸^ORCID,Hayashi Katsuhiko¹^ORCID

Affiliation:

1. Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.

2. Laboratory for Cellular Function Conversion Technology, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan.

3. Functional Genomics, Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, 230-0045, Japan.

4. RNA Biology and Epigenomics Team/LMCP, Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima City, Hiroshima 739-8511, Japan.

5. Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Chuo-ku, Kumamoto 860-0811, Japan.

6. Laboratory of Epigenome Dynamics, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.

7. Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka City 812-8582, Japan.

8. Department of Systems Life Sciences, Graduate School of Systems Life Sciences, Kyushu University, Higashi-ku, Fukuoka City 812-8582, Japan.

9. Department of Anatomy, Kawasaki Medical School, Kurashiki City, 701-0192 Okayama Prefecture, Japan.

Abstract

Reconstituting the ovarian follicle Recent advances have enabled the generation of oocytes from pluripotent stem cells in vitro. However, these cells require a somatic environment to develop fully as reproductive cells. Yoshino et al. applied what is known about differentiation processes in vivo to determine a culture condition to differentiate embryonic stem cells into gonadal somatic cell–like cells (see the Perspective by Yang and Ng). When the embryonic stem cell–generated ovarian gonadal tissue was combined with early primordial germ cells or in vitro–derived primordial germ cell–like cells, germ cells developed into viable oocytes within the reconstituted follicles that could be fertilized and result in viable offspring. This system enables an alternative method for mouse gamete production and advances our understanding of mammalian reproduction and development. Science , abe0237, this issue p. eabe0237 ; see also abj8347, p. 282

Funder

Takeda Science Foundation

Ministry of Education, Culture, Sports, Science, and Technology

The Open Philanthropy Project

the Luca Bella Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference43 articles.

1. Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics

2. Reconstitution in vitro of the entire cycle of the mouse female germ line