Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation-Reference-Cited by-同舟云学术

Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation

Published:2018-06-29 Issue:6396 Volume:360 Page:1423-1429
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Bai Rui¹^ORCID,Wan Ruixue¹^ORCID,Yan Chuangye¹^ORCID,Lei Jianlin¹²^ORCID,Shi Yigong¹³^ORCID

Affiliation:

1. Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Schools of Life Sciences and Medicine, Tsinghua University, Beijing 100084, China.

2. Technology Center for Protein Sciences, Ministry of Education Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

3. Institute of Biology, Westlake Institute for Advanced Study, Westlake University, 18 Shilongshan Road, Xihu District, Hangzhou 310064, Zhejiang Province, China.

Abstract

Structural basis for spliceosome assembly The spliceosome removes noncoding sequences from precursor RNA and ligates coding sequences into useful mRNA. The pre-spliceosome (A complex) associates with a small nuclear ribonucleoprotein (snRNP) complex called U4/U6.U5 tri-snRNP to form the pre-B complex, which is converted into the precatalytic B complex. Bai et al. solved the cryo–electron microscopy structures of the pre-B and B complexes isolated from yeast. These structures show the U1 and U2 snRNPs and allow modeling of the A complex to reveal the early steps of spliceosome assembly and activation. Science , this issue p. 1423

Funder

National Natural Science Foundation of China

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference59 articles.

1. Spliceosome Structure and Function

2. Functional roles of DExD/H-box RNA helicases in Pre-mRNA splicing

3. Structure, function and regulation of spliceosomal RNA helicases

4. A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity

5. An RNA Switch at the 5′ Splice Site Requires ATP and the DEAD Box Protein Prp28p

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