Thymine Dimerization in DNA Is an Ultrafast Photoreaction

Author:

Schreier Wolfgang J.123,Schrader Tobias E.123,Koller Florian O.123,Gilch Peter123,Crespo-Hernández Carlos E.123,Swaminathan Vijay N.123,Carell Thomas123,Zinth Wolfgang123,Kohler Bern123

Affiliation:

1. Department für Physik, Ludwig-Maximilians-Universität, Oettingenstrasse 67, D-80538 München, Germany.

2. Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA.

3. Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, D-81377 München, Germany.

Abstract

Femtosecond time-resolved infrared spectroscopy was used to study the formation of cyclobutane dimers in the all-thymine oligodeoxynucleotide (dT) 18 by ultraviolet light at 272 nanometers. The appearance of marker bands in the time-resolved spectra indicates that the dimers are fully formed ∼1 picosecond after ultraviolet excitation. The ultrafast appearance of this mutagenic photolesion points to an excited-state reaction that is approximately barrierless for bases that are properly oriented at the instant of light absorption. The low quantum yield of this photoreaction is proposed to result from infrequent conformational states in the unexcited polymer, revealing a strong link between conformation before light absorption and photodamage.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Cited by 479 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3