De novo design of protein logic gates-Reference-Cited by-同舟云学术

De novo design of protein logic gates

Published:2020-04-03 Issue:6486 Volume:368 Page:78-84
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Chen Zibo¹²^ORCID,Kibler Ryan D.¹²^ORCID,Hunt Andrew³^ORCID,Busch Florian⁴⁵^ORCID,Pearl Jocelynn⁶,Jia Mengxuan⁴⁵^ORCID,VanAernum Zachary L.⁴⁵^ORCID,Wicky Basile I. M.¹²^ORCID,Dods Galen⁷^ORCID,Liao Hanna⁶^ORCID,Wilken Matthew S.⁶,Ciarlo Christie⁶,Green Shon⁶,El-Samad Hana⁷⁸^ORCID,Stamatoyannopoulos John⁶⁹¹⁰^ORCID,Wysocki Vicki H.⁴⁵^ORCID,Jewett Michael C.³¹¹¹²^ORCID,Boyken Scott E.¹²^ORCID,Baker David¹²¹³^ORCID

Affiliation:

1. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

2. Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.

3. Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA.

4. Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.

5. Resource for Native Mass Spectrometry Guided Structural Biology, The Ohio State University, Columbus, OH 43210, USA.

6. Altius Institute for Biomedical Sciences, Seattle, WA 98195, USA.

7. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

8. Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA.

9. Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.

10. Department of Medicine, Division of Oncology, University of Washington, Seattle, WA 98109, USA.

11. Chemistry of Life Processes Institute, Northwestern University, Evanston, IL 60208, USA.

12. Center for Synthetic Biology, Northwestern University, Evanston, IL 60208, USA.

13. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Abstract

Designer gates Signaling in cells can occur through protein-protein interactions. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a similar structure but where one module can be designed to interact specifically with another module. Using monomers and covalently connected monomers as inputs and encoding specificity through designed hydrogen-bond networks allowed the construction of two-input or three-input gates based on competitive binding. The modular control elements were used to regulate the association of elements of transcription machinery and split enzymes in vitro and in yeast cells. Science , this issue p. 78

Funder

National Institutes of Health

David and Lucile Packard Foundation

Howard Hughes Medical Institute

Army Research Office

Defense Advanced Research Projects Agency

Air Force Research Laboratory

Université du Luxembourg

Schmidt Futures

NIH BTRR Yeast Resource Grant

Camille Dreyfus Teacher Scholar Program

GSK Fellowship