Structural basis of the nucleosome transition during RNA polymerase II passage-Reference-Cited by-同舟云学术

Structural basis of the nucleosome transition during RNA polymerase II passage

Published:2018-11-02 Issue:6414 Volume:362 Page:595-598
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Kujirai Tomoya¹²^ORCID,Ehara Haruhiko²^ORCID,Fujino Yuka¹³,Shirouzu Mikako²^ORCID,Sekine Shun-ichi²^ORCID,Kurumizaka Hitoshi¹²³^ORCID

Affiliation:

1. Laboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.

2. RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.

3. Graduate School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.

Abstract

Nucleosomal DNA transcription In eukaryotes, the basic chromatin unit nucleosome stalls RNA polymerase II (RNAPII) when it transcribes genetic information on DNA. Using cryo–electron microscopy, Kujirai et al. explored seven structures of the RNAPII-nucleosome complex, in which RNAPII pauses at four locations on the nucleosome. These serial snapshots of the RNAPII progression reveal the molecular mechanism of how RNAPII peels the nucleosomal DNA off the histone stepwise. Science , this issue p. 595

Funder

JSPS

JST CREST

RIKEN Dynamic Structural Biology project

Platform Project for Supporting Drug Discovery and Life Science Research (BINDS) from AMED

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference47 articles.

1. A. Wolffe Chromatin: Structure and Function (Academic Press ed. 3 1998).

2. Crystal structure of the nucleosome core particle at 2.8 Å resolution