Role of Bacterial Intimin in Colonic Hyperplasia and Inflammation

Author:

Higgins Lisa M.1,Frankel Gad2,Connerton Ian3,Gonçalves Nathalie S.1,Dougan Gordon2,MacDonald Thomas T.1

Affiliation:

1. Department of Paediatric Gastroenterology, St. Bartholomews and the Royal London School of Medicine and Dentistry, London EC1A 7BE, UK.

2. Department of Biochemistry, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, UK.

3. School of Biological Sciences, Division of Food Sciences, University of Nottingham, LE12 5RD, UK.

Abstract

Enteropathogenic Escherichia coli (EPEC) cells adhere to gut epithelial cells through intimin α: the ligand for a bacterially derived epithelial transmembrane protein called the translocated intimin receptor. Citrobacter rodentium colonizes the mouse colon in a similar fashion and uses a different intimin: intimin β. Intimin α was found to costimulate submitogenic signals through the T cell receptor. Dead intimin β + C. rodentium , intimin α–transfected C. rodentium or E. coli strain K12, and EPEC induced mucosal hyperplasia identical to that caused by C. rodentium live infection, as well as a massive T helper cell–type 1 immune response in the colonic mucosa. Mutation of cysteine-937 of intimin to alanine reduced costimulatory activity in vitro and prevented immunopathology in vivo. The mucosal changes elicited by C. rodentium were interferon-γ–dependent. Immunopathology induced by intimin enables the bacteria to promote conditions that are favorable for increased microbial colonization.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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