Single-Cell DNA-Methylation Analysis Reveals Epigenetic Chimerism in Preimplantation Embryos-Reference-Cited by-同舟云学术

Single-Cell DNA-Methylation Analysis Reveals Epigenetic Chimerism in Preimplantation Embryos

Published:2013-09-06 Issue:6150 Volume:341 Page:1110-1112
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Lorthongpanich Chanchao¹,Cheow Lih Feng²,Balu Sathish¹,Quake Stephen R.²³,Knowles Barbara B.¹⁴,Burkholder William F.²⁵⁶,Solter Davor¹⁷,Messerschmidt Daniel M.¹⁸

Affiliation:

1. Mammalian Development Group, Institute of Medical Biology (IMB), A*STAR, Singapore.

2. Microfluidics Systems Biology Lab, Institute of Molecular and Cell Biology (IMCB), A*STAR, Singapore.

3. Department of Bioengineering and Department of Applied Physics, Stanford University and Howard Hughes Medical Institute, Stanford, CA, USA.

4. The Jackson Laboratory, Bar Harbor, ME, USA.

5. Genome Institute of Singapore, A*STAR, Singapore.

6. Nanyang Technological University, Singapore.

7. Duke–National University of Singapore, Graduate Medical School, Singapore.

8. Developmental Epigenetics and Disease, IMCB, A*STAR, Singapore.

Abstract

Fatal Chimeras Impaired DNA-methylation maintenance during early embryonic development may cause imprinting-related diseases. Lorthongpanich et al. (p. 1110 ) have devised a sensitive assay to probe multiple imprinted gene loci for their DNA-methylation state at the single-cell level. Blastomeres with defective imprinting showed complex, epigenetic chimeras developed with fatal defects. Pronuclear transfer restored normal mouse development, offering a therapeutic strategy to overcome epigenetic defects caused by maternal insufficiencies.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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