Biosensor reveals multiple sources for mitochondrial NAD +

Author:

Cambronne Xiaolu A.1,Stewart Melissa L.1,Kim DongHo1,Jones-Brunette Amber M.2,Morgan Rory K.3,Farrens David L.2,Cohen Michael S.3,Goodman Richard H.1

Affiliation:

1. Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA.

2. Department of Biochemistry and Molecular Biology, Oregon Health & Science University, Portland, OR 97239, USA.

3. Department of Physiology and Pharmacology, Oregon Health & Science University, Portland, OR 97239, USA.

Abstract

A fluorescent sensor for NAD + in living cells Roles of cellular nicotinamide adenine dinucleotide (NAD + ) in metabolism, aging, and disease have garnered much interest, but methods have been lacking to measure the amounts of NAD + in living cells. Cambronne et al. developed a genetically encoded biosensor that can be used to monitor concentrations of free NAD + in various compartments of a cell (see the Perspective by Guarente). Such concentrations of NAD + appear to be important in regulating the activity of NAD + -consuming enzymes such as sirtuins and ADP-ribosyltransferases. The authors used the sensor to demonstrate that NAD + concentrations in mitochondria of cultured human cells can be controlled by multiple mechanisms. Science , this issue p. 1474 ; see also p. 1396

Funder

Pew Charitable Trust

NIH

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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