Promotion of NEDD8-CUL1 Conjugate Cleavage by COP9 Signalosome

Author:

Lyapina Svetlana1,Cope Gregory1,Shevchenko Anna2,Serino Giovanna3,Tsuge Tomohiko3,Zhou Chunshui4,Wolf Dieter A.4,Wei Ning3,Shevchenko Andrej2,Deshaies Raymond J.15

Affiliation:

1. Department of Biology,

2. Protein & Peptide Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69012 Heidelberg, Germany.

3. Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208104, New Haven, CT 06520, USA.

4. Department of Cancer Cell Biology, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA.

5. Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how SCF complexes are regulated, we sought proteins that interact with the human SCF component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated with multiple cullins and promoted cleavage of the ubiquitin-like protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo and in vitro. Multiple NEDD8-modified proteins uniquely accumulated in CSN-deficient S. pombe cells. We propose that the broad spectrum of activities previously attributed to CSN subunits—including repression of photomorphogenesis, activation of JUN, and activation of p27 nuclear export—underscores the importance of dynamic cycles of NEDD8 attachment and removal in biological regulation.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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