Structure of the HIV-1 RNA packaging signal-Reference-Cited by-同舟云学术

Structure of the HIV-1 RNA packaging signal

Published:2015-05-22 Issue:6237 Volume:348 Page:917-921
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Keane Sarah C.¹,Heng Xiao¹,Lu Kun¹,Kharytonchyk Siarhei²,Ramakrishnan Venkateswaran¹,Carter Gregory¹,Barton Shawn¹,Hosic Azra¹,Florwick Alyssa¹,Santos Justin¹,Bolden Nicholas C.¹,McCowin Sayo¹,Case David A.³,Johnson Bruce A.⁴,Salemi Marco⁵,Telesnitsky Alice²,Summers Michael F.¹

Affiliation:

1. Howard Hughes Medical Institute (HHMI) and Department of Chemistry and Biochemistry, University of Maryland Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, MD 21250, USA.

2. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-5620, USA.

3. Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.

4. One Moon Scientific, Incorporated, 839 Grant Avenue, Westfield, NJ 07090, USA, and City University of New York (CUNY) Advanced Science Research Center, 85 St. Nicholas Terrace, New York, NY 10031, USA.

5. Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, and Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA.

Abstract

Structural signals that direct HIV packaging During the viral replication cycle of HIV, unspliced dimeric RNA genomes are efficiently packaged into new virions at the host cell membrane. Packaging is directed by a region at the start of the genome, the 5′ leader. The architecture of the 5′ leader remains controversial. Keane et al. developed nuclear magnetic resonance methods to determine the structure of a 155-nucleotide-long region of the 5′ leader that can direct viral packaging. The structure shows how the 5′ leader binds to the HIV protein that directs packaging, how unspliced dimeric genomes are selected for packaging, and how translation is suppressed when the genome dimerizes. Science , this issue p. 917

Funder

National Institute of General Medical Sciences

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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