Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain-Reference-Cited by-同舟云学术

Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain

Published:2016-06-24 Issue:6293 Volume:352 Page:1586-1590
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Lake Blue B.¹,Ai Rizi²,Kaeser Gwendolyn E.³⁴,Salathia Neeraj S.⁵,Yung Yun C.³,Liu Rui¹,Wildberg Andre²,Gao Derek¹,Fung Ho-Lim¹,Chen Song¹,Vijayaraghavan Raakhee⁵,Wong Julian³,Chen Allison³,Sheng Xiaoyan³,Kaper Fiona⁵,Shen Richard⁵,Ronaghi Mostafa⁵,Fan Jian-Bing⁵,Wang Wei²,Chun Jerold³,Zhang Kun¹

Affiliation:

1. Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

2. Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.

3. Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA, USA.

4. Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, USA.

5. Illumina, San Diego, CA, USA.

Abstract

Single-nucleus gene expression Identifying the genes expressed at the level of a single cell nucleus can better help us understand the human brain. Blue et al. developed a single-nuclei sequencing technique, which they applied to cells in classically defined Brodmann areas from a postmortem brain. Clustering of gene expression showed concordance with the area of origin and defining 16 neuronal subtypes. Both excitatory and inhibitory neuronal subtypes show regional variations that define distinct cortical areas and exhibit how gene expression clusters may distinguish between distinct cortical areas. This method opens the door to widespread sampling of the genes expressed in a diseased brain and other tissues of interest. Science , this issue p. 1586

Funder

NIH Common Fund Single Cell Analysis Program

Neuroplasticity of Aging

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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