Differentiation of Embryonic Stem Cell Lines Generated from Adult Somatic Cells by Nuclear Transfer-Reference-Cited by-全球学者库

Differentiation of Embryonic Stem Cell Lines Generated from Adult Somatic Cells by Nuclear Transfer

Published:2001-04-27 Issue:5517 Volume:292 Page:740-743
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Wakayama Teruhiko¹,Tabar Viviane²,Rodriguez Ivan¹,Perry Anthony C. F.¹,Studer Lorenz²,Mombaerts Peter¹

Affiliation:

1. The Rockefeller University, New York, NY 10021, USA.

2. Laboratory of Stem Cell and Tumor Biology, Neurosurgery and Cellular Biochemistry and Biophysics, Sloan Kettering, New York, NY 10021, USA.

Abstract

Embryonic stem (ES) cells are fully pluripotent in that they can differentiate into all cell types, including gametes. We have derived 35 ES cell lines via nuclear transfer (ntES cell lines) from adult mouse somatic cells of inbred, hybrid, and mutant strains. ntES cells contributed to an extensive variety of cell types, including dopaminergic and serotonergic neurons in vitro and germ cells in vivo. Cloning by transfer of ntES cell nuclei could result in normal development of fertile adults. These studies demonstrate the full pluripotency of ntES cells.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference31 articles.

1. Stem cells — hype and hope

2. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei

3. Cloning of male mice from adult tail-tip cells

4. Mice cloned from embryonic stem cells

5. Mouse strains were B6D2F 1 (C57BL/6 × DBA/2) 129/SvTac 129F 1 (129/SvTac × B6D2F 1 ) nude (C57BL/6 nu/nu ) and EGFP Tg (B6D2F 2 × ICR F 6 ). Mice 8 to 15 weeks old were used as nucleus donors with recipient oocytes from 8- to 10-week-old B6D2F 1 s. Cumulus cells were acutely isolated immediately before nuclear transfer as described previously (2). Tail tip nucleus donors were from 5- to 7-day-old primary cultures presumed to be fibroblasts (3).

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