Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery-Reference-Cited by-同舟云学术

Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery

Published:2021-08-20 Issue:6557 Volume:373 Page:882-889
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Segel Michael¹²³⁴⁵^ORCID,Lash Blake¹²³⁴⁵^ORCID,Song Jingwei¹²³⁴⁵^ORCID,Ladha Alim¹²³⁴⁵^ORCID,Liu Catherine C.¹²³⁴⁵^ORCID,Jin Xin²³⁴⁶^ORCID,Mekhedov Sergei L.⁷,Macrae Rhiannon K.¹²³⁴⁵^ORCID,Koonin Eugene V.⁷^ORCID,Zhang Feng¹²³⁴⁵^ORCID

Affiliation:

1. Howard Hughes Medical Institute, Cambridge, MA 02139, USA.

2. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

3. McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4. Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

5. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

6. Society of Fellows, Harvard University, Cambridge, MA 02138 USA.

7. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.

Abstract

Hitching a ride with a retroelement Retroviruses and retroelements have inserted their genetic code into mammalian genomes throughout evolution. Although many of these integrated virus-like sequences pose a threat to genomic integrity, some have been retooled by mammalian cells to perform essential roles in development. Segel et al . found that one of these retroviral-like proteins, PEG10, directly binds to and secretes its own mRNA in extracellular virus–like capsids. These virus-like particles were then pseudotyped with fusogens to deliver functional mRNA cargos to mammalian cells. This potentially provides an endogenous vector for RNA-based gene therapy. —DJ

Funder

National Institutes of Health

Howard Hughes Medical Institute

G Harold and Leila Y. Mathers Foundation

Edward Mallinckrodt, Jr. Foundation

Open Philanthropy Project

Hock E. Tan and K. Lisa Yang Center for Autism Research at MIT

Poitras Center for Psychiatric Disorders Research at MIT

Yang-Tan Center for Molecular Therapeutics at MIT

Simons Center for the Social Brain at MIT

Publisher

American Association for the Advancement of Science (AAAS)