Optimization of Virulence Functions Through Glucosylation of Shigella LPS-Reference-Cited by-同舟云学术

Optimization of Virulence Functions Through Glucosylation of Shigella LPS

Published:2005-02-25 Issue:5713 Volume:307 Page:1313-1317
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

West Nicholas P.¹²³⁴,Sansonetti Philippe¹²³⁴,Mounier Joëlle¹²³⁴,Exley Rachel M.¹²³⁴,Parsot Claude¹²³⁴,Guadagnini Stéphanie¹²³⁴,Prévost Marie-Christine¹²³⁴,Prochnicka-Chalufour Ada¹²³⁴,Delepierre Muriel¹²³⁴,Tanguy Myriam¹²³⁴,Tang Christoph M.¹²³⁴

Affiliation:

1. Centre for Molecular Microbiology and Infection, Department of Infectious Diseases, Faculty of Medicine, Flowers Building, Imperial College London, London SW7 2AZ, UK

2. Unité de Pathogénie Microbienne Moléculaire, Unité INSERM 389, Institut Pasteur, 28 rue du Dr. Roux, F-75724 Paris Cédex 15, France.

3. Plate-Forme de Microscopie Electronique, Institut Pasteur, 28 rue du Dr. Roux, F-75724 Paris Cédex 15, France.

4. Unité de Résonance Magnétique Nucléaire des Biomolécules, URA 2185 CNRS; Unité INSERM 389, Institut Pasteur, 28 rue du Dr. Roux, F-75724 Paris Cédex 15, France.

Abstract

Shigella , the leading cause of bacillary dysentery, uses a type III secretion system (TTSS) to inject proteins into human cells, leading to bacterial invasion and a vigorous inflammatory response. The bacterium is protected against the response by the O antigen of lipopolysaccharide (LPS) on its surface. We show that bacteriophage-encoded glucosylation of Shigella O antigen, the basis of different serotypes, shortens the LPS molecule by around half. This enhances TTSS function without compromising the protective properties of the LPS. Thus, LPS glucosylation promotes bacterial invasion and evasion of innate immunity, which may have contributed to the emergence of serotype diversity in Shigella .

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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