Aptamer–field-effect transistors overcome Debye length limitations for small-molecule sensing-Reference-Cited by-同舟云学术

Aptamer–field-effect transistors overcome Debye length limitations for small-molecule sensing

Published:2018-10-19 Issue:6412 Volume:362 Page:319-324
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Nakatsuka Nako¹²^ORCID,Yang Kyung-Ae³^ORCID,Abendroth John M.¹²^ORCID,Cheung Kevin M.¹²^ORCID,Xu Xiaobin¹²^ORCID,Yang Hongyan⁴,Zhao Chuanzhen¹²^ORCID,Zhu Bowen¹⁵^ORCID,Rim You Seung¹⁵^ORCID,Yang Yang¹⁵^ORCID,Weiss Paul S.¹²⁵^ORCID,Stojanović Milan N.³⁶^ORCID,Andrews Anne M.¹²⁴^ORCID

Affiliation:

1. California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA.

2. Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.

3. Center for Innovative Diagnostic and Therapeutic Approaches, Department of Medicine, Columbia University, New York, NY 10032, USA.

4. Department of Psychiatry and Biobehavioral Science, Semel Institute for Neuroscience and Human Behavior, and Hatos Center for Neuropharmacology, University of California, Los Angeles, CA 90095, USA.

5. Department of Materials Science and Engineering, University of California, Los Angeles, CA 90095, USA.

6. Departments of Biomedical Engineering and Systems Biology, Columbia University, New York, NY 10032, USA.

Abstract

Transistor sensing in salt solutions Molecular binding to receptors on the surface of field-effect transistors (FETs) can be sensed through changes in transconductance. However, the saline solutions typically used with biomolecules create an electrical double layer that masks any events that occur within about 1 nanometer from the surface. Nakatsuka et al. overcame this limitation by using binding to large, negatively charged DNA stem loop structures that, upon ligand binding, cause conformational changes that can be sensed with an FET, even in solutions with high ionic strength. The authors demonstrate the sensing of charged molecules such as dopamine in artificial cerebrospinal fluid as well as neutral molecules such as glucose and zwitterion molecules like sphingosine-1-phosphate. Science , this issue p. 319

Funder

National Science Foundation

National Institutes of Health

Cal-BRAIN

National Cancer Institute

National Institute of Diabetes and Digestive and Kidney Diseases

Hewlett Packard

Nantworks

Merkin Family Foundation

China Scholarship Council

Publisher

American Association for the Advancement of Science (AAAS)