Affiliation:
1. Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Abstract
The development of resistance is the main threat to the long-term use of toxins from
Bacillus thuringiensis
(Bt) in transgenic plants. Here we report the cloning of a Bt toxin resistance gene,
Caenorhabditis elegans bre-5
, which encodes a putative β-1,3-galactosyltransferase. Lack of
bre-5
in the intestine led to resistance to the Bt toxin Cry5B. Wild-type but not
bre-5
mutant animals were found to uptake toxin into their gut cells, consistent with
bre-5
mutants lacking toxin-binding sites on their apical gut.
bre-5
mutants displayed resistance to Cry14A, a Bt toxin lethal to both nematodes and insects; this indicates that resistance by loss of carbohydrate modification is relevant to multiple Bt toxins.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
192 articles.
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