Achieving optical transparency in live animals with absorbing molecules-Reference-Cited by-同舟云学术

Achieving optical transparency in live animals with absorbing molecules

Published:2024-09-06 Issue:6713 Volume:385 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Ou Zihao¹²^ORCID,Duh Yi-Shiou³⁴^ORCID,Rommelfanger Nicholas J.²⁵^ORCID,Keck Carl H. C.¹²^ORCID,Jiang Shan¹²^ORCID,Brinson Kenneth¹²^ORCID,Zhao Su¹²^ORCID,Schmidt Elizabeth L.¹²^ORCID,Wu Xiang¹²^ORCID,Yang Fan¹²^ORCID,Cai Betty¹,Cui Han¹²^ORCID,Qi Wei⁶,Wu Shifu¹²,Tantry Adarsh²⁷^ORCID,Roth Richard⁸^ORCID,Ding Jun⁸⁹^ORCID,Chen Xiaoke⁶^ORCID,Kaltschmidt Julia A.²⁸^ORCID,Brongersma Mark L.¹⁴⁵^ORCID,Hong Guosong¹²^ORCID

Affiliation:

1. Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.

2. Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.

3. Department of Physics, Stanford University, Stanford, CA, USA.

4. Geballe Laboratory for Advanced Materials, Stanford University, Stanford, CA, USA.

5. Department of Applied Physics, Stanford University, Stanford, CA, USA.

6. Department of Biology, Stanford University, Stanford, CA, USA.

7. Neurosciences IDP Graduate program, Stanford University, Stanford, CA.

8. Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA.

9. Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA, USA.

Abstract

Optical imaging plays a central role in biology and medicine but is hindered by light scattering in live tissue. We report the counterintuitive observation that strongly absorbing molecules can achieve optical transparency in live animals. We explored the physics behind this observation and found that when strongly absorbing molecules dissolve in water, they can modify the refractive index of the aqueous medium through the Kramers-Kronig relations to match that of high-index tissue components such as lipids. We have demonstrated that our straightforward approach can reversibly render a live mouse body transparent to allow visualization of a wide range of deep-seated structures and activities. This work suggests that the search for high-performance optical clearing agents should focus on strongly absorbing molecules.

Publisher

American Association for the Advancement of Science (AAAS)

Link

https://www.science.org/doi/pdf/10.1126/science.adm6869

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