The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells-Reference-Cited by-同舟云学术

The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells

Published:2022-01-28 Issue:6579 Volume:375 Page:411-418
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Melani Rafael D.¹^ORCID,Gerbasi Vincent R.¹^ORCID,Anderson Lissa C.²^ORCID,Sikora Jacek W.¹^ORCID,Toby Timothy K.¹^ORCID,Hutton Josiah E.¹^ORCID,Butcher David S.²^ORCID,Negrão Fernanda¹,Seckler Henrique S.¹,Srzentić Kristina¹,Fornelli Luca¹^ORCID,Camarillo Jeannie M.¹^ORCID,LeDuc Richard D.¹^ORCID,Cesnik Anthony J.³⁴^ORCID,Lundberg Emma³⁴^ORCID,Greer Joseph B.¹^ORCID,Fellers Ryan T.¹^ORCID,Robey Matthew T.¹^ORCID,DeHart Caroline J.¹^ORCID,Forte Eleonora⁵⁶,Hendrickson Christopher L.²^ORCID,Abbatiello Susan E.⁷^ORCID,Thomas Paul M.¹^ORCID,Kokaji Andy I.⁸,Levitsky Josh⁹^ORCID,Kelleher Neil L.¹¹⁰^ORCID

Affiliation:

1. Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.

2. National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA.

3. Department of Genetics, Stanford University, Stanford, CA, USA.

4. Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH–Royal Institute of Technology, Stockholm, Sweden.

5. Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.

6. Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

7. Thermo Fisher Scientific, Cambridge, MA, USA.

8. STEMCELL Technologies Inc., Vancouver, BC, Canada.

9. Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

10. Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Abstract

Human biology is tightly linked to proteins, yet most measurements do not precisely determine alternatively spliced sequences or posttranslational modifications. Here, we present the primary structures of ~30,000 unique proteoforms, nearly 10 times more than in previous studies, expressed from 1690 human genes across 21 cell types and plasma from human blood and bone marrow. The results, compiled in the Blood Proteoform Atlas (BPA), indicate that proteoforms better describe protein-level biology and are more specific indicators of differentiation than their corresponding proteins, which are more broadly expressed across cell types. We demonstrate the potential for clinical application, by interrogating the BPA in the context of liver transplantation and identifying cell and proteoform signatures that distinguish normal graft function from acute rejection and other causes of graft dysfunction.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference76 articles.

1. A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis

2. Impact of regulatory variation from RNA to protein

3. A draft map of the human proteome

4. Mass-spectrometry-based draft of the human proteome

5. A subcellular map of the human proteome

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