Affiliation:
1. Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg 12 rue du Général Zimmer, 67084 Strasbourg cedex, France.
2. Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331, USA.
Abstract
siRNA Movement in Plant Tissues
Long-distance movement of RNA interference (RNAi)–derived signals in plants plays an important role in development and in defense against viral attack. The nature of the signals that spread from cell to cell is not known, although evidence suggests that they are nucleic acids of some sort (see the Perspective by
Martienssen
).
Molnar
et al.
(p.
872
, published online 22 April) and
Dunoyer
et al.
(p.
912
, published online 22 April) now show that in
Arabidopsis
, both exogenous and endogenous small interfering RNAs (siRNAs), rather than their long double-stranded precursor RNAs, are the molecules that transfer information between plant cells. A viral protein that counters RNAi though sequestering siRNAs blocked spreading of a transgene RNAi silencing signal. Furthermore, siRNA-processing enzymes were required in the source, and not the recipient, cells for spreading, and bombardment of plants with double-stranded siRNAs directly showed siRNA spread between cells. Endogenous siRNAs also spread between tissues and were capable of directing DNA methylation of target sequences in distant tissues.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
308 articles.
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