Structure and Asn-Pro-Phe Binding Pocket of the Eps15 Homology Domain-Reference-Cited by-同舟云学术

Structure and Asn-Pro-Phe Binding Pocket of the Eps15 Homology Domain

Published:1998-08-28 Issue:5381 Volume:281 Page:1357-1360
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

de Beer Tonny¹,Carter Royston E.¹,Lobel-Rice Katherine E.¹,Sorkin Alexander¹,Overduin Michael¹

Affiliation:

1. Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA.

Abstract

Eps15 homology (EH) domains are eukaryotic signaling modules that recognize proteins containing Asn-Pro-Phe (NPF) sequences. The structure of the central EH domain of Eps15 has been solved by heteronuclear magnetic resonance spectroscopy. The fold consists of a pair of EF hand motifs, the second of which binds tightly to calcium. The NPF peptide is bound in a hydrophobic pocket between two α helices, and binding is mediated by a critical aromatic interaction as revealed by structure-based mutagenesis. The fold is predicted to be highly conserved among 30 identified EH domains and provides a structural basis for defining EH-mediated events in protein trafficking and growth factor signaling.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference41 articles.

1. Signaling Through Scaffold, Anchoring, and Adaptor Proteins

2. Fazioli F., Minichiello L., Matoskova B., Wong W. T., Di Fiore P. P., Mol. Cell. Biol. 13, 5814 (1993);

3. Wong W. T., et al., Proc. Natl. Acad. Sci. U.S.A. 92, 9530 (1995) .

4. Salcini A. E., et al., Genes Dev. 11, 2239 (1997).

5. M. Yamabhai et al. in preparation.

Cited by 114 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The tandem EH domains of End3 cooperate to interact with dual XPF motifs of Sla1 for the connection of early and late stages in fungal endocytosis;Biochemical and Biophysical Research Communications;2023-06

2. Completing the family of human Eps15 homology domains: Solution structure of the internal Eps15 homology domain of γ‐synergin;Protein Science;2022-01-12

3. Up-Regulation of the Long Noncoding RNA X-Inactive–Specific Transcript and the Sex Bias in Pulmonary Arterial Hypertension;The American Journal of Pathology;2021-06

4. Protein Interaction Domains: Structural Features and Drug Discovery Applications (Part 2);Current Medicinal Chemistry;2021-04-01

5. Sex differences in the proliferation of pulmonary artery endothelial cells: implications for plexiform arteriopathy;Journal of Cell Science;2020-05-01