Antimicrobial Activity of Typha angustata Bory and Chaub Inflorescence Against Wound Associated Bacteria

Abstract

Microbes are gaining resistance against chemotherapeutic agents (mostly antibiotics). Thus in recent times it has become very essential to search relatively safe, cheap and effective therapeutics from the plant source. The objective of the current study is to determine the antibacterial activity of Typha angustata Bory and Chaub. aqueous crude extract against bacteria isolated from the patients having different types of wound. A total of 50 clinical samples were obtained from patients having a wound, after their consent. Twenty isolates of pure bacterial cultures were detected. Escherichia sp. was found to be the predominant agent isolated from the wound infections (30%) followed by Staphylococcus spp. (25%), Streptococcus spp (25%), and Pseudomonas sp., (20%). Sensitivity of the bacterial isolates were tested against selected antibiotics: CIP- ciprofloxacin; OFX- oflaxacin; CN- gentamycin; P- penicillin; OX-oxacillin; E- erythromycin, PB- polymyxin B LEV- levofloxacin; OFX- oflaxacin ; AMP- ampicillin; and TE-tetracyclin. The antimicrobial activity of T. angustata inflorescence crude extract was determined by well diffusion method. The results revealed that inhibition zone for Staphylococcus spp isolates were found to be in the range of (13mm-19mm), Streptococcus sp isolates (16mm 19mm), Psuedomonas sp. (18mm-20mm) and Escherichia sp. (16mm-19mm). The Minimum Inhibitary Concentration (MIC) and Minimum Bacterial Concentration (MBC) values of T. angustata inflorescence crude extract against isolated species from wound samples were recorded in the range of (30-120mg/ml). The results of this study indicate that T. angustata has potential to be developed into antibacterial agent against resistant and susceptible bacteria that are mainly responsible for causing wound infections.