Effect of light Intensity and Temperature on in vitro Somatic Embryos Germination Stage in Date Palm

Author:

Shehata Wael F. H.1,Belal Abdel Fatah H.2,El-Deeb Mohamed D.2

Affiliation:

1. Plant Production Department, College of Environmental Agricultural Science, El–Arish University, North Sinai, Arab Republic of Egypt and Agricultural Biotechnology Department, College of Agricultural and Food Sciences, King Faisal University, Hassa, Saudi Arabia.

2. Plant Production Department, College of Environmental Agricultural Science, El–Arish University, North Sinai, Arab Republic of Egypt

Abstract

The aim of this study is to detect the extent of physical factors (light intensity and temperature) effect on proliferation and germination of date palm somatic embryos in vitro. The treatments of this study were carried out by using two degrees of temperature (18 ± 2 ºC & 28 ± 2 ºC) with four levels of light intensity (0, 20, 40 and 60 μmol m-2 s-1). The obtained results revealed the following: The results showed that there are significant differences between the eight treatments of physical factors under study as the results monitored that the highest average for No. of somatic embryos germination was for the explants cultured at light intensity of 40 μmol m-2 s-1 and under low temperature of 18±2ºC, whereas the highest average for germination lengths was for the explants cultured at light intensity of 40 μmol m-2 s-1 and high temperature of 28±2ºC all over the study. While, the lowest values were for the explants cultured at total darkness.

Publisher

Emerald

Subject

General Medicine

Reference25 articles.

1. Abul-Soad, A.A. and Mahdi, S.M. (2010). Commercial production of tissue culture date palm (Phoenix dactylifera L.) by inflorescence technique. Journal of Genetic Engineering and Biotechnology. 8 (2), pp. 39- 44.

2. Alkhateeb, A.A. (2008). Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology. 99: 6550-6555.

3. Al-Khayri, J.M. (2003). In vitro germination of somatic embryos in date palm: effect of auxin concentration and strength of MS salts. Current Science. 84: 680- 683.

4. Al-Khayri, J.M. (2001). Optimization of biotin and thiamine requirements for somatic embryogenesis of date palm (Phoenix dactylifera L.). In vitro Cell. Dev. Biol. Plant. 37: 453-456.

5. Al-Khayri, J.M. (2011). Basal salt requirements differ according to culture stage and cultivar in date palm somatic embryogenesis. Am. J. Biochem. Biotechnol. 7: 32-42.

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3