Optimization of the Culture Medium for the Production of Intracellular β-Galactosidase from Kluyveromyces marxianus

Abstract

This paper investigates the optimization of the production of β-Galactosidase using a yeast universal medium containing lactose by Kluyveromyces marxianus in shake flask cultures at 35C, 125 rpm and PH=5. Fermentation technology in the shake flask culture was used to investigate the effect the supplements, including (Trace elements, Isopropyl β-D-1-thiogalactopyranoside (IPTG) and thiamin vitamin), had on β-Galactosidase enzyme production by Kluyveromyces marxianus. The supplements were separately added to the universal medium of yeast containing lactose. Results of the statistical analysis showed that among the different examined media: supplementing the medium with both (1% trace elements and 1% thiamin vitamin) has a significant effect on ß-Gal production at the 5% significance level respectively.