The relationship of polymorphisms of the SOD2, NOS3 and SIRT1 genes with hypertension in employees of the converter workshop of the ferrous metallurgy enterprise

Abstract

Workers employed in ferrous metallurgy enterprises are affected by various oxidants, which lead to increased formation of free radicals, suppression of the antioxidant system, initiation of oxidative stress and the development of diseases characterized by high blood pressure. In this regard, the identification of polymorphisms of genes encoding enzymes that are associated with the formation and neutralization of free radicals is an important task of personalized medicine. The study aims to explore the relationship of polymorphisms rs4880 (SOD2), rs1799983 (NOS3) and rs7069102 (SIRT1) with hypertension in workers of the converter workshop. To conduct the research the authors used data from periodic medical examinations of men aged 30 to 59 years, including 116 employees of the converter shop and 169 representatives of administrative and managerial personnel (comparison group). Subsequently, we divided each group according to the principle of the presence or absence of a diagnosis of "hypertensive heart disease" (I11 according to ICD-10), established or confirmed during a periodic medical examination. DNA was isolated from peripheral blood using the Lumiere kit for the isolation of genomic DNA from whole blood and literal epithelium (Lumiprobe, Russia) in accordance with the manufacturer's instructions. The scientists performed genotyping using the optical PCR system with real-time optical detection QuantStudio 3 (Thermo Fisher, USA) and ready-made commercial sets "SNP-Screen" (Syntol, Russia). The ratio of the chances of developing hypertension in the personnel of the converter shop was increased in carriers of mutant genotypes of the SOD2 (TT) gene. Also, the values of blood pressure, total cholesterol and glucose levels were higher in carriers of the mutant TT genotype of the SOD2 gene compared with CC/CT, but only in the group of converter shop workers with hypertension. In addition, in the comparison group with hypertension, systolic and diastolic blood pressure significantly differed, the values of which were higher in carriers of the mutant GG genotype of the SIRT1 gene. Presumably, an excess of superoxide anion in the TT genotype of the SOD2 gene, together with exposure to harmful environmental factors, may contribute to the development of cardiovascular diseases in workers of the converter workshop. Ethics. The study was approved in accordance with Protocol No. 1 dated 02/26/2021 and the conclusion of the Local Ethics Committee of the Federal Budgetary Institution of Science "Yekaterinburg Medical Scientific Center for the Prevention and Health Protection of Industrial Workers" of Rospotrebnadzor.