Internal Temperatures of Packaging for Overnight Cross-country Shipping of Zebrafish (Danio Rerio)

Author:

Fehrenbach Logan A1,Adekanye Adewole A2,Palillo Michael B3,Bidot Willie A4,Malbrue Raphael A2,Coble Dondrae J2,Kendall Genevieve C5

Affiliation:

1. Animal Resources Core, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio;, Email: Logan.Fehrenbach@nationwidechildrens.org

2. Animal Resources Core, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio

3. Department of Preventative Medicine, The Ohio State University, Columbus, Ohio

4. Office of Animal Resources, Western University of Health Sciences, Pomona, California

5. Center for Childhood Cancer and Blood Diseases, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio; Department of Pediatrics, The Ohio State University, Columbus, Ohio

Abstract

As the use of zebrafish (Danio rerio) as a research model continues to rise, so too will the shipping and sharing of zebrafish strains across collaborating institutions. If done incorrectly, shipping can result in significant mortality, welfare concerns, and loss of valuable resources for researchers and research institutions. Here we introduce a novel method to track temperatures of zebrafish containers during shipping and show that internal packaging temperatures are directly affected by the external temperatures. We used temperature logging Thermochron iButtons to track the temperatures of 2 packages containing adult zebrafish that were shipped overnight from Dallas, TX to Columbus, OH during winter following recommended fish shipping guidelines. We found that the external packaging of both boxes of fish were exposed to temperatures that had previously been shown to be lethal to zebrafish. However, internal temperatures and, more specifically, water temperature, stayed within 24.0 to 26.5°C during shipment, resulting in 100% survival of adult zebrafish. This novel method of tracking packaging temperatures of live fish during shipping can help to inform fish health status on arrival.

Publisher

American Association for Laboratory Animal Science

Subject

General Veterinary,General Biochemistry, Genetics and Molecular Biology

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