Doğrulama istenen idrarda madde analiz sonuçlarının incelenmesi

Abstract

Objective: This study aims to investigate the false positive outcomes in urine analysis via the immunoassay method within an addiction treatment center outpatient clinic. While widely utilized for substance detection, false positive results in the immunoassay method can cause misleading. This study aims to attract attention to false positivity and its implications. Methods: Conducted at an Alcohol and Drug Addiction Center outpatient clinic, the study retrospectively examines urine analyses from February to May 2023. Among 5109 immunoassay-based urine analyses, only 25 were subjected to confirmation through liquid chromatography-tandem mass spectrometry (LC-MS). Results: The findings demonstrate that LC-MS verified 40% of immunoassay-positive urine samples as true positives. Significantly, false positive results were notable, particularly in cases involving benzodiazepines. The study accentuates a noteworthy disparity between initial immunoassay outcomes and subsequent confirmatory tests, casting doubts on the reliability of the immunoassay method. A fundamental discovery is the consistent identification of pregabalin and gabapentin in urine samples yielding false positive benzodiazepine results during confirmation analysis. This revelation prompts inquiries into the potential cross-reactivity of these medications in immunoassay-based tests, suggesting the need for careful consideration in clinical and forensic contexts. Conclusion: The study underscores the importance of confirmatory testing for result accuracy and the multifaceted implications of false positives on patient-doctor relationships, treatment decisions, and patient safety. Acknowledging the study's limitations, such as its retrospective nature and limited participant pool, the research underscores the requirement for a comprehensive approach to substance detection, merging screening and confirmatory analyses to enhance diagnostic dependability.