The means of combating persistent infection of viral diarrhea

Abstract

The paper presents results of research into the means of inhibiting persistent infection caused by noncytopathic biotype of bovine viral diarrhea virus. It was shown that bovine viral diarrhea virus, widespread in the cattle population in the whole world, can be a primary cause of pathology development of the respiratory and reproductive organs, and can cause a persistent form of infection in animals. The virus can contaminate fetal serum, cell cultures, trypsin, and other biological products, which leads to a decrease in the quality of biotech products from these materials and the spread of bovine viral diarrhea virus in a population of susceptible animals. The study was done on the model of a continuous calf coronary cell culture persistently infected with the noncytopathic biotype of the bovine viral diarrhea virus. The antiviral effect of two commercial preparations Ribavirin-Lipint and Reaferon-EC-Lipint was studied. The effectiveness of antiviral treatment of the infected cell culture was determined by the reverse transcription polymerase chain reaction, carried out in two versions: with electrophoresis and in real-time mode. It was found that these drugs in doses of 0.05 mg / ml and 30000 IU / ml, respectively, when added to growth medium for 24 consecutive passages, led to a decrease in the infectious activity of the bovine viral diarrhea virus in the cell culture, but did not cure it completely from the persistent infection caused by the noncytopathic biotype of the virus. Application of antiviral drugs is one of the ways to decrease economic losses caused by biological product contamination, the use of persistently infected animals for pedigree purposes and restrictions in international cattle trade.