Spectrophotometric Estimation of Flutamide in Pure and in Pharmaceutical Preparations

Abstract

Three simple, rapid, precise, sensitive, and accurate visible spectrophotometric methods (A, B, and C) are described for the estimation of flutamide in both pure and pharmaceutical preparations. They are based on the diazotization of reduced flutamide (FA) with nitrous acid followed by coupling with acetyl acetone (method A), citrazinic acid (method B), and 8-hydroxyquinoline (method C) to form colored azo dyes, exhibiting absorption maxima at 410, 440 and 500 nm, for methods A, B, and C, respectively. Linear concentration range was of 0.5–12, 0.5–10, and 0.3–10 μg/mL for methods A, B, and C, respectively, and the corresponding molar absorptivity values are , , and  L mol−1 cm−1. The correlation coefficients for FA are 0.9988, 0.9976, and 0.9975 for methods A, B, and C, respectively. All variables were optimized, and the results were statistically compared with those of a literature method by employing Student’s t-test and F-test. No interference was observed from common adjuvants normally added to the tablets. The relative standard deviations () for FA in all the three methods in the tablets are always less than 3%.