Use of Bacterial Artificial Chromosomes in Baculovirus Research and Recombinant Protein Expression: Current Trends and Future Perspectives

Author:

Roy Polly1,Noad Rob2ORCID

Affiliation:

1. Department of Pathogen Molecular Biology, Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK

2. Centre for Emerging Endemic and Exotic Diseases, Department of Pathology and Infectious Diseases, Royal Veterinary College, Hatfield AL9 7TA, UK

Abstract

The baculovirus expression system is one of the most successful and widely used eukaryotic protein expression methods. This short review will summarise the role of bacterial artificial chromosomes (BACS) as an enabling technology for the modification of the virus genome. For many years baculovirus genomes have been maintained inE. colias bacterial artificial chromosomes, and foreign genes have been inserted using a transposition-based system. However, with recent advances in molecular biology techniques, particularly targeting reverse engineering of the baculovirus genome by recombineering, new frontiers in protein expression are being addressed. In particular, BACs have facilitated the propagation of disabled virus genomes that allow high throughput protein expression. Furthermore, improvement in the selection of recombinant viral genomes inserted into BACS has enabled the expression of multiprotein complexes by iterative recombineering of the baculovirus genome.

Publisher

Hindawi Limited

Subject

Management, Monitoring, Policy and Law,Geography, Planning and Development

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