Author:
Iqbal Asfand,Khursid Rabia,Fatima Nida,Rizwan Muhammad
Abstract
Natural plant extracts are valuable source of numerous fungitoxic compounds that can substitute synthetic fungicides. In current studies, six plant extracts viz., Zingiber officinalis, Eucalyptus camaldulensis, Azadirachta indica, Allium cepa, Cassia fistula and Allium sativum in three dose levels standard dose (S.D), S/2 and S/3 were tested against colony growth of Macrophomina phaseolina under in vitro conditions. Among all the treatments ginger extract at its standard dose was found highly effective followed by eucalyptus and neem. Percentage colony growth inhibition of (50 %), (38 %) and (29 %) was recorded to produce by ginger, eucalyptus and ginger respectively as compared to control treatment where no growth inhibition was recorded. Extract of Allium sativum was found least effective phytochemical where pathogenic fungal growth inhibition was (24.5 %). Concentration comparison of three dose levels showed that (S.D) was highly effective where mean colony growth of M. phaseolina was (3.72 cm) followed by (4 cm) @ S/2 and maximum diameter was (4.36 cm) @ S/3 concentration.
Reference31 articles.
1. Abid, M., S. Ehtheshamul-Haque, M.A. Maqbool and A. Ghaffar. 1992. Effect of oil cakes, Bradyrhizobium sp., Paecilomyces lilacinus and furdan on root nodulation and root-knot nematodes in mung bean. Pakistan Journal of Nematology, 10: 145-150.
2. Agrios, G.N. 2005. Plant pathology fifth edition. Ed Elsevier Academia Press. San Diego Calf. USA.
3. Akhtar, M.S. and Z.A. Siddiqui. 2008. Arbuscular mycorrhizal fungi as potential bio-protectants against plant pathogens. Mycorrhizae: Sustainable Agriculture and Forestry, 61–97.
4. Bajwa, R., N. Akhtar and Bashir. 2001. Antifungal activity of allelopathic plant extracts. Effect of allelopathic plant extracts of three allelopathtic Asteracous species on the growth of Apergilli. Pakistan Journal of Biological Sciences, 33:105-107.
5. Bylka, W., M.S. Hajdyrch, I. Matalawska and O. Goslinka. 2004. Anti-microbial activity of isocytisocide and extracts of Aquilegia vulgaris L. Letters in Applied Microbiology, 39: 93-97.