Alteration in Biochemical Responses in Leaves of Potato due to Common Scab Disease

Abstract

The current study was designed at the research area of Plant Pathology Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan during 2017– 2020. Fresh leaves of inoculated and un-inoculated potato varieties highly susceptible (FD 76-36), moderately susceptible (FD 73-110), and resistant (Esmee)/ test lines were collected during 2017-18 and 2018-19, at 35, 65 and 95 days after sowing for biochemical analysis.  Superoxide dismutase activity, Catalase activity, Per-oxidase activity, Protein and Total phenolics contents were quantified. FD 76-36 exhibited minimum SOD activity (67.733, 45.637, 24.910) %, followed by FD 73-110 (70.303,57.893, 42.513) % and Esmee (84.567, 65.167, 45.873) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum CAT activity (10.990, 7.473, 3.413) %, followed by Esmee (13.537, 9.630, 8.147) % and FD 73-110 (19.277, 8.147, 6.170) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 exhibited minimum POD activity (0.2133, 0.2767, 0.3600) %, followed by FD 73-110 (0.2900, 0.5567, 0.6300) % and Esmee (0.8800, 1.2733, 1.5433) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum protein contents (3.097, 2.873, 1.260) %, followed by FD 73-110 (7.907, 5.423, 4.267) % and Esmee (12.163, 8.633, 5.127) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum TPC (136.31, 115.58, 70.77) %, followed by FD 73-110 (165.85, 136.75, 86.89) % and Esmee (188.42, 158.38, 109.00) % after 35, 65 and 95 days of inoculation respectively as compared to control. The experiment was conducted with a randomized complete block design (RCBD) and three replications. The experimental data were analyzed using Fisher’s analysis of variance technique and treatment means were compared by the least significance difference (LSD) test at a 5% probability level.