Hematological Changes in Stored Citrate Phosphate Dextrose Adenine Bag Blood

Abstract

OBJECTIVES: This study aims to find the efficacy of stored whole blood for a period of 49 days and to delineate the changes that occur in Haemoglobin (HB), Red Blood Cell (RBC) and White Blood Cell (WBC) indices and Platelet count. METHODOLOGY: The study was carried out at District Headquarter (DHQ) Hospital, District Hangu in collaboration with the blood bank unit. 450 ml of blood was drawn from 10 healthy volunteer donors into an anticoagulant blood bag (CPDA-1) (63 mL). Blood bags were carefully stored in a quarantine shelf of the blood bank at 02-08°C. Samples were collected and tested for various haematological parameters (haemoglobin, RBC count, WBC count, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets count) at days 01 and 49 respectively on (ADVIA 360 haematology analyser). RESULTS: Statistically significant changes were observed in WBC count, Lymphocyte count and platelets count and gradual changes in mean corpuscular volume. While statistically non-significant changes were observed in other parameters (RBC, haemoglobin, haematocrit, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration).   CONCLUSION:   Haemolysis of the red cells that occurs during component processing and storage of red cell units has serious clinical implications for the blood recipient patients. Detecting excessive haemolysis important to minimize transfusion of bacterially contaminated blood units. Rapid degeneration of leukocytes could lead to immunomodulation related to blood transfusion. Whole blood should be leuko-depleted before storage if it must be used beyond one week.