Detection of yellow mosaic virus resistance in Soybean (Glycine max L.) genotypes for yield and related traits

Abstract

Background Twenty soybean genotypes were screened for Soybean yellow mosaic virus (YMV) identification which were collected from the Genetics and plant breeding farm laboratory. Methods To evaluate genetic studies of the chosen materials, the use of a net house assay upon sap inoculation made it easier to determine the trend of the Area Under Disease Progress Curve (AUDPC) in a controlled environment. Younger leaves from these plants were gathered and used for molecular analysis. Results Six genotypes were identified with semi-resistant disease reactions. Yellow mosaic virus-resistant and susceptible soybean genotypes were distinguished using a soybean “Rsv1-h” gene-based primer pair. To discover the existence of the Rsv-1-h gene responsible for YMV resistance, a genetic diversity panel of 15 soybean genotypes was investigated with two simple sequence repeat (SSR) markers. The BARCSOYSSR_13_1115 and BARCSOYSSR_13_1173 primer sets were employed to validate the desired genes in these genotypes. Conclusion The genotypes HIHS-WIHS, MINA-HAI, Shohag, G-2120, BRAGG, and BS-3 presented the major gene Rsv1-h, which is said to be essential for yellow mosaic virus resistance and can provide better yield also than other genotypes. The six soybean genotypes with the Rsv1-h gene may be resistant to mosaic virus and could be exploited as a source of improved breeding material in the future.