Degree of organ damage and inflammatory markers in sepsis mice models inducted by various doses of lipopolysaccharides

Abstract

Background: Sepsis is a life-threatening disease, and animal models of sepsis are minimal. This study aims to find the optimal dose to make a sepsis mouse model by examining the presence of target organ damage. Methods: This study used 30 mice divided into four groups. The control group injected 0.5 cc NaCl 0.9% intraperitoneally (i.p.). Group A was injected with lipopolysaccharides (LPS) 0.25 mg/kg B.W. i.p. given on the first and second day, group B was injected with LPS 0.3 mg/kg B.W. i.p. given on the first and second days, and group C was injected by LPS 0.3 mg/kg B.W. single dose i.p. Each group was terminated on the third, fourth, and fifth days. Results: NF-κB, C-reactive protein (CRP), alanine aminotransferase (ALT), NF-κB examinations, and tumor necrosis factor-α (TNF-α) in all treatment groups increased when compared with the control. The highest degree of histopathological features of the kidneys and liver and the results of immunohistochemistry examinations on the liver and kidneys were shown in group C. Conclusions: Inflammatory markers (CRP, TNF-α, NF-κB, and expression of NF-κB in liver and kidneys) and characteristics of organ damage (ALT, liver, and kidneys histopathology scores) increase on day 3. The highest increase was in the group administered with LPS 0.3 mg/kg B.W. single dose.