Abstract
There continues to be no approved drugs for the treatment of Ebola virus disease (EVD). Despite a number of candidate drugs showing limited efficacy in vitro and/or in non-human primate studies, EVD continues to plaque certain areas of Africa without any efficacious treatments yet available. Recently, we have been exploring the potential for anti-malarial drugs to inhibit an in vitro model of Ebola Zaire replication using a transcription-competent virus-like particle (trVLP) assay. We examined the efficacy of chloroquine, amodiaquine and 36 novel anti-parasite quinoline derivatives at inhibiting Ebola virus replication. Drug efficacy was tested by trVLP assay and toxicity by MTT assay. Both chloroquine and amodiaquine were effective for inhibition of Ebola virus replication without significant toxicity. The half-maximal inhibitory concentration (IC50) of chloroquine and amodiaquine to inhibit Ebola virus replication were IC50, Chl = 3.95 µM and IC50, Amo = 1.45 µM, respectively. Additionally, three novel quinoline derivatives were identified as having inhibitory activity and low toxicity for Ebola trVLP replication, with 2NH2Q being the most promising derivative, with an IC50 of 4.66 µM. Quinoline compounds offer many advantages for disease treatment in tropical climates as they are cheap to produce, easy to synthesize and chemically stable. In this report, we have demonstrated the potential of anti-parasite quinolines for further investigation for use in EVD.
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference33 articles.
1. WHO | Ebola virus disease.,2018
2. Ebola | Ebola situation reports: Democratic Republic of the Congo.,2019
3. The Ebola outbreak, 2013-2016: old lessons for new epidemics.;C Coltart;Philos Trans R Soc Lond B Biol Sci.,2017
4. Immunomonitoring of human responses to the rVSV-ZEBOV Ebola vaccine.;D Medaglini;Curr Opin Virol.,2017
5. Transmission of ebola virus disease: an overview.;S Rewar;Ann Glob Heal.,2014
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献