Active compound test: ethanolic extract of White Oyster Mushroom (Pleurotus ostreatus) Using HPLC and LC-MS

Abstract

Background: The use of herbs as traditional medicine in Indonesia is increasing, with more than 40% of Indonesia's population utilizing them. White oyster mushroom (Pleurotus Ostreatus) is a fungus that has various therapeutic effects including antioxidant, anti-inflammatory, anti-bacterial, anti-cholesterol, and anti-cancer properties. This mushroom contains many active substances in its secondary metabolites which have pharmacological effects. The purpose of this study was to identify the active compounds in the ethanolic extract of white oyster mushroom that will form the extract profile and become the basis for drug development. Methods: The active compound test used High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography with Mass Spectrometer (LC-MS). Ethanolic extract of white oyster mushroom was processed by 70% alcohol maceration, evaporation, and thickening. Results: The results of the HPLC test showed that the ethanolic extract of white oyster mushroom contained cinnamic acid and rutin, while the LC-MS test showed the presence of p-coumaric acid, Ascorbic acid, Linoleic acid, 9-Eicosene (E), Niacinamide, Veritric acid, Syringic acid, Ergosterol. Conclusions: Cinnamic acid, rutin, p-coumaric acid, Ascorbic acid, Linoleic acid, 9-Eicosene (E), Niacinamide, Veratric acid, Syringic acid, and Ergosterol that were detected in the ethanolic extract of white oyster mushroom showed that the extract had the potential for antioxidant and anti-inflammatory activity.