Abstract
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP that is robust, reliable, repeatable, specific, and inexpensive.
Funder
Medical Research Council
European Molecular Biology Organization
H2020 Marie Skłodowska-Curie Actions
Cancer Research UK
Rosetrees Trust
The John Black Charitable Foundation
Wellcome Trust
Subject
General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Reference24 articles.
1. A Novel Coronavirus from Patients with Pneumonia in China, 2019.;N Zhu;N Engl J Med.,2020
2. A new coronavirus associated with human respiratory disease in China.;F Wu;Nature.,2020
3. A pneumonia outbreak associated with a new coronavirus of probable bat origin.;P Zhou;Nature.,2020
4. Laboratory testing of SARS-CoV, MERS-CoV, and SARS-CoV-2 (2019-nCoV): Current status, challenges, and countermeasures.;Y Yan;Rev Med Virol.,2020
5. Scalable and Resilient SARS-CoV-2 testing in an Academic Centre.;J Aitken;Health Systems and Quality Improvement.,2020