SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay

Author:

Buck Michael D.ORCID,Poirier Enzo Z.ORCID,Cardoso Ana,Frederico Bruno,Canton Johnathan,Barrell Sam,Beale Rupert,Byrne Richard,Caidan Simon,Crawford Margaret,Cubitt LauraORCID,Gandhi Sonia,Goldstone Robert,Grant Paul R.,Gulati Kiran,Hindmarsh Steve,Howell Michael,Hubank Michael,Instrell RachaelORCID,Jiang Ming,Kassiotis GeorgeORCID,Lu Wei-TingORCID,MacRae James I.ORCID,Martini Iana,Miller Davin,Moore David,Nastouli Eleni,Nicod JeromeORCID,Nightingale Luke,Olsen Jessica,Oomatia Amin,O'Reilly Nicola,Rideg Anett,Song Ok-Ryul,Strange Amy,Swanton Charles,Turajlic Samra,Wu Mary,Reis e Sousa CaetanoORCID,

Abstract

The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP that is robust, reliable, repeatable, specific, and inexpensive.

Funder

Medical Research Council

European Molecular Biology Organization

H2020 Marie Skłodowska-Curie Actions

Cancer Research UK

Rosetrees Trust

The John Black Charitable Foundation

Wellcome Trust

Publisher

F1000 Research Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

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