Behavioral response of the small hive beetle, Aethina tumida (Coleoptera: Nitidulidae) to volatiles of Apicure®, a plant-based extract

Abstract

Background: The small hive beetle (SHB), Aethina tumida is an invasive pest of the honey bee. Although no previous methods have led to its successful management, yeast inoculated pollen baited-traps have showed promise as quick monitoring tools. In this study, we evaluated the role of olfaction in SHB response to Apicure®, an essential oil-based biopesticide that has shown potential for the management of honey bee pests and diseases. Methods: Volatiles from Apicure® were collected using super Q adsorbent traps. Subsequent analysis was done using Gas chromatography- mass spectrophotometry (GC-MS) to ascertain the components of Apicure®. The selectivity and sensitivity of antennal receptors of A. tumida adults to the volatile compounds were determined using behavioral assays and Gas Chromatography-Electroantennodetection (GC-EAD). Results: GC-MS analysis showed that Apicure® consists of 40 compounds. GC-EAD analysis isolated 11 compounds that elicited antennal response with the SHB. Of these, linalool, camphor, geraniol and α-terpineol were confirmed to be strongly repellant, while limonene was attractive to SHB in dual-choice olfactometer assays. Conclusion: Our results demonstrate that the major components in Apicure® are mainly repellants thus prospective in disrupting the host recognition by the SHB. The product therefore can be up-scaled for the management of SHB.