Abstract
Conducting collaborative and comprehensive epidemiological research on neonatal sepsis in low- and middle-income countries (LMICs) is challenging due to a lack of diagnostic tests. This prospective study protocol aims to obtain epidemiological data on bacterial sepsis in newborns and young infants at Kamuzu Central Hospital in Lilongwe, Malawi. The main goal is to determine if the use of whole blood transcriptome host immune response signatures can help in the identification of infants who have sepsis of bacterial causes. The protocol includes a detailed clinical assessment with vital sign measurements, strict aseptic blood culture protocol with state-of-the-art microbial analyses and RNA-sequencing and metagenomics evaluations of host responses and pathogens, respectively. We also discuss the directions of a brief analysis plan for RNA sequencing data. This study will provide robust epidemiological data for sepsis in neonates and young infants in a setting where sepsis confers an inordinate burden of disease.
Subject
Public Health, Environmental and Occupational Health,Health Policy,Immunology and Microbiology (miscellaneous),Biochemistry, Genetics and Molecular Biology (miscellaneous),Medicine (miscellaneous)
Reference42 articles.
1. Global, regional, and national sepsis incidence and mortality, 1990-2017: analysis for the Global Burden of Disease Study.;K Rudd;Lancet.,2020
2. The global burden of paediatric and neonatal sepsis: a systematic review.;C Fleischmann-Struzek;Lancet Respir Med.,2018
3. Global, regional, and national causes of under-5 mortality in 2000-15: an updated systematic analysis with implications for the Sustainable Development Goals.;L Liu;Lancet.,2016
4. Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000.;L Liu;Lancet.,2012
5. Pediatric sepsis in the developing world.;N Kissoon;J Infect.,2015
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