Tumor cell-secreted soluble uPAR functions as a neutrophil chemoattractant to promote triple-negative breast cancer metastasis

Author:

liu xia1ORCID,LI Dong,hemati hami,Younhee Park,Taftaf Rokana,zhang youbin,Liu Jinpeng1,Cristofanilli Massimo

Affiliation:

1. University of Kentucky

Abstract

Abstract Accumulating evidence have demonstrated that circulating tumor cell (CTC) clusters have higher metastatic ability than single CTCs, and correlates with worse cancer patient’s outcomes. The heterotypic CTC clusters such as neutrophil-CTC clusters recently were identified in both cancer mouse models and cancer patients, leading to more efficient metastasis formation compared with homotypic CTC clusters. However, the mechanism by which neutrophils are associated with CTCs remains elusive. In this study, we found that the intercellular adhesion molecule (ICAM-1) on triple-negative breast cancer (TNBC) cells mediates their binding with CD11b+ neutrophils, and CD11b deficiency inhibited TNBC metastasis In vivo. Additionally, CD11b mediated H2O2 production of neutrophils. Further studies indicated that ICAM-1 promotes uPAR secretion, which functions as a chemoattractant for neutrophils. Knockdown of uPAR in ICAM-1+ TNBC cells reduced lung-infiltrating neutrophils, and lung metastasis. The bioinformatics analysis showed uPAR is highly expressed in TNBCs, which positively correlates with higher neutrophil infiltration and negatively with breast cancer patient’s survival. Together, our findings discover a novel chemoattractant role of suPAR in TNBC metastasis, and provides a rationale for targeting ICAM-1-uPAR-CD11b axis to block CTC-neutrophil cluster formation, and its-mediated metastasis.

Publisher

Research Square Platform LLC

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3