Identification, Prevalence and Susceptibility Profile of Candida Isolates at the Pasteur Institute in Côte D'ivoire From 2017 to 2019-Reference-Cited by-同舟云学术

Identification, Prevalence and Susceptibility Profile of Candida Isolates at the Pasteur Institute in Côte D'ivoire From 2017 to 2019

Published:2022-08-09 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Ira Valerie Bonouman¹^ORCID,M'Boh Reine²,Roger Frédéric³,Krasteva Donika³,Koffi David²,Noell Julie³,Pottier Cyrille³,Bellet Virginie³,Drakulvoski Pascal³,Menan Hervé⁴,Touré Offianan André²,Dosso Mireille²,Ranque Stéphane⁵,Bertout Sébastien³

Affiliation:

1. Université de Montpellier Faculté de Pharmacie: Universite de Montpellier Faculte de Pharmacie

2. Institut Pasteur de Côte d'Ivoire: Institut Pasteur de Cote d'Ivoire

3. Universite de Montpellier UFR des Sciences Pharmaceutiques et Biologiques: Universite de Montpellier Faculte de Pharmacie

4. CHU de Treichville: Centre Hospitalier Universitaire de Treichville

5. IHU Méditerranée Infection: IHU Mediterranee Infection

Abstract

Abstract Aims: This study was conducted to determine the respective prevalence of Candida species and to study their sensitivity profile to antifungal agents to identify the emergence of resistance.Methods: This retrospective study was conducted from 2017 to 2019 at the Institute Pasteur of Côte d’Ivoire on patients with fungal disease. Various samples were analysed first by conventional methods. Then, the results were confirmed by MALDI-TOF-MS. Sensitivity tests were performed using the CLSI method and evaluated by the M59 benchmark.Results: In total, 227 Candida species were isolated from 1966 patients. Most of the samples were vaginal swabs (120/228). Using the conventional method, C. albicans was prevalent, being present in 52.6% of the samples. There were mixtures of 2 to 4 species in 36.8% of the samples that were not identified at the first identification. Confirmation by MALDI-TOF-MS showed a 35.18% prevalence of C. tropicalis. Thirteen species were identified, and 2 species complexes (C. albicans and C. parapsilosis) were highlighted in a final cohort of 315 Candida strains.Conclusion: This study highlights the difficulty of correct identification in developing countries. The transition from standard identification to molecular biology is essential and must be considered for improvement in public health.

Publisher

Research Square Platform LLC

Reference38 articles.

1. Fontecha G, Kathy M, Bryan O, Celeste G, Sharleen B Identification of Cryptic Species of Four Candida Complexes in a Culture Collection. J Fungi 2019, 5, 117 7 of 12. 10.3390/jof5040117.

2. Molecular identification and antifungal susceptibility profile of yeast from vulvovaginal candidiasis;Shi Y;BMC Infect Dis,2020

3. Ngouana TK, Krasteva D, Drakulovski P, Toghueo RK, Kouanfack C, Ambe A, Reynes J, Delaporte E, Boyom FF, Mallie M, et al. Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaounde (Cameroon) HIV-infected patients. Mycoses 2015, 58, 33–3. DOI: 10.1111/myc.12266.

4. A Re-Evaluation of the Relationship between Morphology and Pathogenicity in Candida species;Kadosh D;J Fungi,2020

5. Ghazi S, Rafei R, Osman M, Safadi DE, Mallat H, Papon N, Daboussi F, Bouchara JP. M. Hamze. The epidemiology of Candida species in the Middle East and North Africa. Mycologie médicale. https//doi.org/10.1016/j.mycmed.2019.07.006.