Alkaloid-Metabolomics & DNA profiles: Role-bioassay-guided differentiation process of Six Annona cultivated in Egypt on anti-cancer therapy

Abstract

Abstract Trees of the Annona species that grow in the tropics and subtropics contain compounds that are highly valuable for pharmacological research and medication development and have anticancer, antioxidant, and migratory properties. Metabolomics was used to functionally characterize natural products and to distinguish differences between varieties. Natural products are therefore bioactively marked and highly respected in the field of drug innovation. Our study was aimed to assess the inter-relationships among six Annona sp. Using six SCoT and six ISSRs primers taken for DNA fingerprinting we found polymorphism of 45.16 and 35.29%, respectively. The GC-MS-based plant metabolomics was employed to compare profiles of 78 different volatile oil compounds in six Annona sp. and 74 characterized alkaloids compound metabolomics bioassay-guided differentiation process by structural analysis through HPLC-ESI-MSn, UPLC-HESI-MS/MS as antiproliferative activities of five in vitro cell lines. Metabolomics based on high throughput and low sensitive LC/MS enabled the in-depth studies on changes of secondary metabolites with bioassay-guided differentiation process exanimate in the cytotoxic, antioxidant and migration activities of twenty four extract prepared from six Annona sp. were evaluated in vitro. In vitro testing on cell cultures showed a significant concentration- cytotoxic effect on all cell lines (HepG2, HCT, Caco, Mcf-7, and T47D) treated at exposure time (48 h) with six volatile oil samples. Therefore they showed remarkable antioxidant activity simultaneously with a cytotoxic effect. Also the samples which have a significant IC50 showed a considerable effect to stop the metastasis and invasion of different cancer cell lines which makes it an interesting subject for further research.