Detection of Vancomycin- resistant S. aureus (VRSA) producing van A gene in Khartoum state, Sudan

Abstract

Abstract Background: Staphylococcus aureus is a major causative agent of bacterial infection in Sudan. Resistance to antibiotic has increased dramatically over the past few years and has now researched a level that place patient in real danger. This study aimed to detect the resistance patterns of Staphylococcus aureusisolates, MDR and Vancomycin Resistance Staphylococcus aureus that produced Van A gene. This study included 87 staphylococcus aureus isolates. The detection of the resistance isolates done by using different types of antimicrobial agents included as Oxacillin (1mcg), Penicillin (10mcg), Colistin (10mcg), Amoxicillin-clavulanic acid (10mcg), Ciprofloxacin (5mcg) and Vancomycin (30mcg). Then the PCR technique was done for Van A gene detection. Results: In this study isolates of S. aureus show that the highest resistance rate was reported among Penicillin 87(100%), Oxacillin 87(100%) which represents the prevalence of MRSA and Amoxicillin- clavulanic acid 87(100%). The isolates revealed elevated resistance rate for Colistin 79(90.8%). The prevalence of Vancomycin Resistant 9(10.3%). The prevalence of Multidrug-Drug Resistance Staphylococcus aureus isolates was 36(41.4%),2 (22.2%) of VRSA isolates were positive to Van A gene. Conclusions: Methicillin resistance increases time to time rapidly. Vancomycin still a choice of treatment for Staphylococcus aureus infections.