LINC1521 and miR-3679-5p modulate cellular response to chemical stress in breast cancer patients through regulation of XBP1 expression as a potential diagnostic biomarker

Abstract

Abstract Background The majority of breast cancer (BC) patients die of metastasis rather than primary tumors, whereas the molecular mechanisms orchestrating cancer metastasis remains poorly understood. Long noncoding RNAs (lncRNA) have been shown to regulate cancer occurrence and progression. However, the lncRNAs that drive metastasis in cancer patients and their underlying mechanisms are still largely unknown. In this study, expression, interaction, and related pathways of XBP1 and a novel regulatory non-coding RNA, LINC01521 is evaluated in BC patients. Methods Microarray data analysis was performed using R Studio (4.1.2). GSE71052 was used in this study. miRNA interaction analysis carried out using miRWalk. lncRNA-mRNA interaction analysis was conducted using lncRRIsearch. Expression and survival analysis was performed by GEPIA2. Illustration of interaction analyses was performed by cytoscape. The qRT-PCR experiment was performed for the validation of expression results. Results XBP1 (logFC:, p-value: ) and LINC01521 (logFC:, p-value: ) have significant up-regulation in BC and could be the two potential diagnostic biomarker of BC. Up-regulation of XBP1 observed in bioinformatics analyses and supported by experimental results in this study. XBP1 regulates the function of HMOX1 in cellular response to chemical stress signaling pathway. miR-3679-5p suppresses the expression level of XBP1 (energy: -30.5, score: 1, position: 3’UTR). XBP1 also involved in Response To Interleukin-4 (GO:0070670) and Positive Regulation Of B Cell Differentiation (GO:0045579) biological processes. Conclusion miR-3679-5p as a novel miRNA and LINC01521 as a potential oncogene and diagnostic biomarker might modulate cellular response to chemical stress signaling pathway in BC patients through regulation of XBP1, a BC oncogene.